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Reprogrammable mouse jaenisch

2020-02-18 00:28

A reprogrammable mouse: the secondary system. Cultivation of these secondary somatic cells in the presence of doxycycline efficiently generated secondary iPS cells, which grew independently of Dox and were shown to be pluripotent by stringent criteria. Two major advantages are offered by this strategy.Here, we used gene targeting to generate a mouse strain with a single copy of an inducible, polycistronic reprogramming cassette, allowing for the induction of pluripotency in various somatic cell types. Since these reprogrammable mice can be easily bred, they provide a useful tool to study the mechanisms underlying cellular reprogramming. reprogrammable mouse jaenisch

Dec 13, 2009  (c) Brightfield (top) and green fluorescence (bottom) images of an iPSC colony derived from postnatal tailtip fibroblasts (TTFs) from a reprogrammable mouse

A reprogrammable mouse strain from genetargeted embryonic stem cells. from a reprogrammable mouse. Rudolf Jaenisch; Proviruses carrying druginducible Oct4, Sox2, Klf4 and cMyc used to Dec 14, 2009 Konrad Hochedlinger, Rudolf Jaenisch, and their respective colleagues now independently report reprogrammable mouse strains that will be useful for this purpose. Both teams introduced the genes that encode the four reprogramming factors as a single set into a defined location in the mouse genome. reprogrammable mouse jaenisch Mouse ES Cells Isolated The interest in stem cells has grown exponentially cells reprogrammable by nuclear cloning? Nuclear Cloning of Terminally rearrangements (Hochedlinger and Jaenisch, 2002) B. CNS: Cloning of postmitotic mature neurons Does brain development neuronal functions involve epigenetic as well as genetic alterations

A reprogrammable mouse strain from genetargeted embryonic stem cells. Nat Methods. 2010 Jan; 7(1): 535. Epub 2009 Dec 13. PubMed PMID: . 7: Stadtfeld M, Apostolou E, Akutsu H, Fukuda A, Follett P, Natesan S, Kono T, Shioda T, Hochedlinger K. Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells. reprogrammable mouse jaenisch Arguably the most widely used reprogrammable mouse model is the m2rtTAOKSM mouse (Stadtfeld et al. , 2010) which harbours (I) a multicistronic OKSM cassette with an inducible promoter at the Collagen1a1 locus, (II) the m2 reverse transactivator (m2rtTA) constitutively expressed from the Rosa26 locus and (III) a GFP reporter (A) The cell sorting analyses of Doxwithdrawn kidney tumor cells in a reprogrammable chimeric mouse with the Lgr5EGFP reporter. GFPpositive Lgr5expressing cells were sorted to exclusively isolate Doxwithdrawn tumor cells. Sep 11, 2013 Abstract. Notably, reprogrammable mice present circulating iPS cells in the blood and, at the transcriptome level, these in vivo generated iPS cells are closer to embryonic stem cells (ES cells) than standard in vitro generated iPS cells. Moreover, in vivo iPS cells efficiently contribute to the trophectoderm lineage, Conversion of somatic cells to pluripotency by defined factors is a long and complex process that yields embryonic stem celllike cells that vary in their developmental potential. To improve the quality of resulting induced pluripotent stem cells (iPSCs), which is important for potential therapeutic

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